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Related Concept Videos

Indicators02:39

Indicators

Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are called...
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Fluorescent dye N,N'-dioctadecylrhodamine as a new interfacial acid-base indicator.

N O McHedlov-Petrossyan1, N A Vodolazkaya, O N Bezkrovnaya

  • 1V.N. Karazin Kharkov National University, 61077 Kharkov, Ukraine. mchedlov@univer.kharkov.ua

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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PubMed
Summary

This study explores the fluorescent dye N,N'-dioctadecylrhodamine, detailing its spectral properties and behavior at interfaces. The dye effectively monitors surface potentials and determines bulk pH in various aqueous environments.

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Area of Science:

  • Physical Chemistry
  • Surface Chemistry
  • Spectroscopy

Background:

  • Fluorescent dyes are crucial for interfacial studies.
  • Understanding protolytic behavior is key to dye applications.
  • N,N'-dioctadecylrhodamine's properties at interfaces are not fully characterized.

Purpose of the Study:

  • To investigate the spectral properties of N,N'-dioctadecylrhodamine.
  • To analyze the protolytic behavior of the dye at interfaces.
  • To evaluate the dye's potential for monitoring surface potentials and pH.

Main Methods:

  • Spectroscopic analysis (absorption and fluorescence).
  • Studies at micelle/water and microdroplet/water interfaces.
  • Langmuir-Blodgett film preparation and analysis in aqueous media.

Main Results:

  • The dye exhibits distinct spectral properties at interfaces.
  • Long hydrocarbon chains dictate dye orientation, directing the dissociating group towards the aqueous phase.
  • Both absorption and fluorescence spectra correlate with electrical surface potentials and bulk pH.

Conclusions:

  • N,N'-dioctadecylrhodamine is a suitable probe for interfacial studies.
  • The dye's spectral changes can be used for real-time monitoring of surface electrical potentials.
  • This fluorescent dye enables accurate determination of bulk pH in aqueous systems.