Misoprostol enhances early fracture healing: a preliminary biochemical study on rats

The purpose of this study was to demonstrate if misoprostol, a methyl derivative of prostaglandin E1, enhanced fracture healing in 54 male adult Sprague-Dawley rats. The base level of serum alkaline phosphatase (ALP) in 6 randomly selected rats was measured. Rats were then randomly separated into 3 groups and their tibias fractured. First and second groups received misoprostol for 4 weeks, 100 and 300 microg/kg/day respectively via oral route. The third group had no misoprostol. p<0.05 was considered significant. Elevation of ALP level in the 2nd week was significant in group 1, it was not in group 2 or 3; in the 4th week it was significant in all groups. In conclusion dosage dependent osteoinductive effect of misoprostol was shown in the early bone healing period. Biochemical findings in the latter period did not show any inhibitory effect of misoprostol on bone healing. Further studies, probably biomechanical, may be required for the final verdict.