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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Gene expression assays.

Janine Kirby1, Paul R Heath, Pamela J Shaw

  • 1Academic Neurology Unit, Section of Neuroscience, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2RX, United Kingdom.

Advances in Clinical Chemistry
|August 9, 2007
PubMed
Summary

Gene expression assays analyze genome-wide gene activity, advancing disease research. These powerful tools offer insights into cellular functions and disease pathogenesis, particularly in cancer and neuroscience.

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Last Updated: Jul 13, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
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Area of Science:

  • Genomics and Molecular Biology
  • Biotechnology

Background:

  • Gene expression profiling provides insights into cellular protein synthesis plans.
  • Technological advancements enable whole-genome expression analysis in single experiments.
  • Gene expression assays have evolved from single-gene measurements to comprehensive genomic analysis.

Purpose of the Study:

  • To outline the development and applications of gene expression assays.
  • To provide an overview of current gene expression technologies.
  • To highlight scientific achievements and future directions in the field.

Main Methods:

  • Genome-wide gene expression analysis.
  • Laser capture microdissection and linear amplification of RNA.
  • Advanced techniques: exon arrays, tiling arrays, and on-chip chromatin immunoprecipitation.

Main Results:

  • Gene expression assays have significantly advanced understanding of disease pathogenesis.
  • Applications highlighted in cancer (e.g., breast cancer) and neuroscience (e.g., Huntington's disease, schizophrenia).
  • Recent technological innovations expand the scope of gene expression analysis.

Conclusions:

  • Gene expression assays are crucial tools in modern biological research.
  • Continued technological advancements promise deeper insights into gene expression complexities.
  • The technology plays a vital role in understanding disease mechanisms and developing potential therapies.