The antimicrobial peptide PR-39 has a protective effect against HeLa cell apoptosis

PR-39 is a cathelicidin with antimicrobial properties, which acts as a suppressor of inflammation and exerts a number of additional activities. We investigated for the first time the effect of PR-39 on human cells, by addressing the possible interference with HeLa cell metabolism. We observed that the continuous administration of PR-39 to HeLa cell cultures was not cytotoxic and did not interfere with DNA synthesis. When used in combination with a panel of drugs inducing apoptosis through different mechanisms of action, i.e. etoposide, bleomycin, tert-butylhydroperoxide and 2-deoxy-d-ribose, PR-39 attenuated the apoptotic response of HeLa cells.