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Updated: Jul 13, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
[Effect of fluorosis on the expression of basic fibroblast growth factor in rat incisors]
Hui Liu1, Qiang Wang, Feng Zhu
1Department of Cariology and Endodontology, School of Stomatology, Tongji University, Shanghai 200072, China.
Objective:
To investigate the effects of overdose fluoride on the expression of basic fibroblast growth factor (bFGF) in rat incisors.
Methods:
Twenty Wistar rats were randomly divided into 2 groups: group I (control group) distilled water was given; group II (experimental group) 100 mg/L NaF was given. The rats were killed at the end of 8 th week. Immunohistochemical staining was used to study the expression of bFGF in rat incisors.
Results:
Immunohistochemical results demonstrated the presence of bFGF in ameloblasts, odontoblasts of rat incisors. The expression of bFGF was reduced in group II (P < 0.01).
Conclusions:
Overdose fluoride inhibits the expression of bFGF and affects the interaction between dental epithelium and dental mesenchyme, which leads to the enamel demineralization.

