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Updated: Aug 2, 2026
Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
Differences in the expression of histocompatibility antigen-DR and in anti-mycobacterial activity of monocytes from
B S Zwilling1, J Salkowitz, H Laufman
1Department of Microbiology, Ohio State University, Columbus.
Abstract:
This study assesses the changes in the expression of histocompatibility antigen (HLA)-DR by mononuclear phagocytes from HIV-infected individuals. Overnight culture of monocytes resulted in an increase in HLA-DR expression by monocytes from uninfected individuals. In contrast, the expression of HLA-DR by monocytes from HIV-infected patients decreased spontaneously and was most pronounced in patients with clinical AIDS. We also found that Mycobacterium avium grew within monocytes from patients infected with HIV. The correlation between major histocompatibility complex (MHC) class II expression and Mycobacterial growth which has been reported in mice was not observed in monocytes from HIV-infected patients.
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