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Related Experiment Video

Updated: Jul 13, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

A compact multiphoton 3D imaging system for recording fast neuronal activity.

Dejan Vucinić1, Terrence J Sejnowski

  • 1Howard Hughes Medical Institute, Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, United States of America. dejan@salk.edu

Plos One
|August 9, 2007
PubMed
Summary

We developed a low-cost, compact two-photon imaging system for fast neuronal activity recording. This system enables high-speed 3D scanning and fluorescence lifetime imaging, integrating seamlessly with electrophysiology setups.

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Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Engineering

Background:

  • Studying fast neuronal activity requires advanced imaging techniques.
  • Existing two-photon microscopy systems can be expensive and complex.
  • There is a need for cost-effective, high-performance imaging solutions for neuroscience research.

Purpose of the Study:

  • To develop a simple, compact, and affordable two-photon imaging system.
  • To achieve high-speed neuronal activity recording in 3D.
  • To enable integration with electrophysiology for simultaneous measurements.

Main Methods:

  • Construction of a system using an Yb:KYW femtosecond laser and acousto-optic deflection.
  • Implementation of a two-axis acousto-optic deflector for 3D targeting.

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Last Updated: Jul 13, 2026

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Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

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  • Utilizing a digital oscilloscope for data acquisition and scan control.
  • Development of open-source software and firmware for system operation.

    • Main Results:

    • Achieved full-frame acquisition at video rates (suitable for patch clamping).
    • Enabled random access in under 10 microseconds with dwelling capability.
    • Demonstrated 3D random access and fast volume sweeps at kilohertz rates.
    • Successfully performed fluorescence lifetime imaging.
    • Recorded action potentials with high temporal resolution using potentiometric dye.

    Conclusions:

    • The developed system offers a cost-effective alternative to conventional two-photon microscopes.
    • Its capabilities include high-speed 3D imaging and fluorescence lifetime measurements.
    • The system's ease of integration with electrophysiology facilitates widespread adoption for studying neuronal activity.