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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Cell Signaling Feedback Loops01:07

Cell Signaling Feedback Loops

Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Positive and Negative Feedback Loops01:18

Positive and Negative Feedback Loops

Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires  maintaining an internal dynamic equilibrium:
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!

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Related Experiment Video

Updated: Jul 13, 2026

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Monitoring Notch1 activity in development: evidence for a feedback regulatory loop.

Gonzalo Del Monte1, Joaquím Grego-Bessa, Alvaro González-Rajal

  • 1Departamento de Inmunología y Oncología, Centro Nacional de Biotecnología/CSIC, Campus Cantoblanco, Madrid, Spain.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|August 10, 2007
PubMed
Summary

This study maps active Notch1 signaling during mouse development, revealing its presence in the mesoderm, heart, and various organs. Notch1 activity appears regulated by a positive feedback loop.

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Last Updated: Jul 13, 2026

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

Area of Science:

  • Developmental Biology
  • Cell Signaling

Background:

  • Notch signaling is crucial for cell fate and embryonic development.
  • Ligand binding triggers Notch receptor cleavage, releasing the active Notch intracellular domain (NICD).

Purpose of the Study:

  • To analyze the distribution of active mouse Notch1 (N1ICD) during specific developmental stages.
  • To investigate the regulatory mechanisms of Notch1 expression.

Main Methods:

  • Utilized an antibody targeting N1ICD.
  • Employed a highly sensitive staining technique for detection.
  • Analyzed Notch1 activity in wild-type and Notch pathway mutant zebrafish and mice.

Main Results:

  • Earliest N1ICD expression detected in mesoderm and developing heart (endocardium, cardiac valves).
  • N1ICD found in presomitic mesoderm during segmentation, arterial endothelium, kidney, pancreas, thymus, CNS, and sensory placodes.
  • Notch1 transcription and activity were significantly reduced in Notch pathway mutants.

Conclusions:

  • Notch1 plays a vital role in multiple embryonic tissues and organogenesis.
  • Vertebrate Notch1 expression is likely regulated by a positive feedback loop, indicating self-amplification.