Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Monitoring Fetal Somatic Cell Genome Editing <i>In Vivo</i> with Total-Body Positron Emission Tomography: Studies in Rhesus Macaques (<i>Macaca mulatta</i>).

Human gene therapy·2026

Same author

A split luciferase biosensing platform for detection and imaging of chromatin loops in individual live cells.

Nucleic acids research·2025

Same author

Hypochlorous Acid-Gated Hydrolysis of a Phosphinate Ester Dye in Living Cells.

Journal of the American Chemical Society·2025

Same author

A Modular Platform for the Optogenetic Control of Small GTPase Activity in Living Cells Reveals Long-Range RhoA Signaling.

bioRxiv : the preprint server for biology·2025

Same author

Split-Small GTPase Reassembly as a Method to Control Cellular Signaling with User-Defined Inputs.

ACS chemical biology·2025

Same author

Engineered Silyl Lipids to Modulate Liposome and Lipid Nanoparticle Properties for mRNA Delivery.

ACS applied bio materials·2025

Search research articles

Related Experiment Video

Updated: Jul 13, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Split beta-lactamase sensor for the sequence-specific detection of DNA methylation.

Jason R Porter¹, Cliff I Stains, David J Segal

¹Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA.

Analytical Chemistry

|August 10, 2007

Summary

A new DNA methylation detection system, mCpG-SEER-beta-Lac, offers enhanced specificity and speed for identifying methylated CpG sites. This advancement aids in early cancer diagnosis and understanding epigenetic regulation.

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Related Experiment Videos

Last Updated: Jul 13, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Area of Science:

Epigenetics
Molecular Biology
Cancer Diagnostics

Background:

DNA methylation at CpG sites is crucial in epigenetics and linked to cancer.
Accurate and rapid detection methods are needed for early cancer diagnosis and epigenetic research.

Purpose of the Study:

To develop a second-generation, highly sensitive DNA methylation detection system.
To improve upon previous split-protein sensor technology for CpG methylation detection.

Main Methods:

Utilized a split enzymatic reporter (TEM-1 beta-lactamase) linked to DNA binding elements.
Developed the mCpG-SEER-beta-Lac system for site-specific detection of DNA methylation.
Assessed specificity for methylated versus non-methylated CpG sites.

Main Results:

The mCpG-SEER-beta-Lac system demonstrated >40-fold specificity for methylated CpG sites.
Achieved sensitive detection of 2.5 fmol methylated target dsDNA in just 5 minutes.
Showcased a 250-fold increase in assay speed and a 2000-fold increase in sensitivity compared to the first-generation system.

Conclusions:

The mCpG-SEER-beta-Lac system provides a sensitive and rapid method for detecting specific DNA methylation.
This technology has potential applications in early cancer diagnostics and epigenetic studies.
The split enzymatic reporter approach significantly enhances DNA methylation detection capabilities.