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Updated: Jul 13, 2026
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
[Screening and expression of tumor markers in colorectal carcinoma]
Jian-Feng Zhang1, Chun-Fang Gao, Xiu-Li Wang
1Research Institute of Anal-Colorectal Surgery, the 150 Center Hospital of the People's Liberation Army, Luoyang 471031, China. zjfhxf@126.com
Objective:
To screen the tumor markers of colorectal carcinoma and to investigate their expression in preoperative and postoperative serum.
Methods:
The distinct proteins in serum were detected in 87 cases of colorectal carcinoma, 68 cases of benign colorectal diseases and 56 healthy people by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The distinct proteins in serum were detected in 87 cases of colorectal carcinoma pre-operation and within 15 days post operation with the same methods.
Results:
Two proteins with mass-to-charge ratios of 5955 Da and 5972 Da were screened. Compared with benign colorectal diseases and healthy control, the expression of the two proteins was obviously up-regulated in colorectal carcinoma (P < 0.01). Compared with pre-operation, the expression on the 1(st) day post operation was obviously up-regulated (P < 0.01), and the expression decreased to pre-operative level on the 4(th) day post operation. The expression of the two proteins turned out to be a descendent tendency form the 4(th) to 15(th) day post operation, but did not reach the normal level as found in healthy control.
Conclusions:
Two proteins with mass-to-charge ratios of 5955 Da and 5972 Da could be regarded as tumor markers in colorectal carcinoma, the expression may has some regularity.
