Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bone Disorders

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Essential Minerals for Bone Health

Essential Minerals for Bone Health

The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

Bone Marrow Sampling and Transplants

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

Bone Remodeling and Repair

Bone Remodeling and Repair

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structure-Based Design of Small-Molecule Inhibitors of Human Interleukin-6.

bioRxiv : the preprint server for biology·2025

Same author

Osteocalcin binds to a GPRC6A Venus fly trap allosteric site to positively modulate GPRC6A signaling.

FASEB bioAdvances·2024

Same author

GPRC6A is a Potential Therapeutic Target for Metformin Regulation of Glucose Homeostasis in Mice.

bioRxiv : the preprint server for biology·2024

Same author

Mechanical protein polycystin-1 directly regulates osteoclastogenesis and bone resorption.

Science bulletin·2024

Same author

Mechanosensitive protein polycystin-1 promotes periosteal stem/progenitor cells osteochondral differentiation in fracture healing.

Theranostics·2024

Same author

Drugging the entire human proteome: Are we there yet?

Drug discovery today·2024

Search research articles

Related Experiment Video

Updated: Jul 13, 2026

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Published on: April 14, 2016

RNA interference and its application in bone-related diseases.

Ya Lin Li¹, L Darryl Quarles, Hong Hao Zhou

¹Institute of Clinical Pharmacology, Central South University, Changsha 410078, China.

Biochemical and Biophysical Research Communications

|August 10, 2007

Summary

RNA interference (RNAi) offers a powerful method for studying gene function by degrading mRNA. This technique is becoming a practical tool for in vitro and in vivo research, particularly for bone-related diseases.

More Related Videos

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

Related Experiment Videos

Last Updated: Jul 13, 2026

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

Published on: April 14, 2016

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

RNA interference (RNAi) has revolutionized gene function studies.
It enables targeted mRNA degradation using small interfering RNAs (siRNAs) or small hairpin RNAs (shRNAs).
RNAi is more efficient and convenient than traditional knockout methods.

Purpose of the Study:

To review recent advancements in RNAi technology.
To explore the development of RNAi as a tool for studying bone-related diseases.

Main Methods:

Focus on recent achievements in RNAi.
Depict the development of RNAi for disease research.

Main Results:

RNAi provides new perspectives for genome-wide gene function surveys.
Enhanced stability and delivery vehicles are improving RNAi's practicality.
RNAi is poised to become a key tool in biological research.

Conclusions:

RNAi is a significant biological insight with broad applications.
Its utility is expanding for in vitro and in vivo studies.
RNAi holds promise for advancing the study of bone-related diseases.