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Updated: May 1, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
Published on: February 21, 2014
Molecular mechanisms of COPII vesicle formation
Marcus C S Lee1, Elizabeth A Miller
1Department of Microbiology, Columbia University, New York, NY, United States.
Abstract:
The first step in protein secretion from eukaryotic cells is mediated by COPII vesicles, known for the cytoplasmic coat proteins that are the minimal machinery required to generate these small transport carriers. The five COPII coat components coordinate to create a vesicle by locally generating membrane curvature and populating the incipient bud with the appropriate cargo. This review describes the molecular details of how the COPII coat sculpts vesicles from the endoplasmic reticulum and highlights some unresolved questions regarding the regulation of this process in the complex environment of the eukaryotic cell.
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