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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
Transgenic Organisms00:53

Transgenic Organisms

Overview
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Updated: Jul 13, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
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pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants.

Claudia E Vickers1, Peer M Schenk, Dongxue Li

  • 1Department of Biological Sciences, The University of Essex, Wivenhoe Park, Colchester, C04 3SQ, England.

Biotechnology Letters
|August 10, 2007
PubMed
Summary
This summary is machine-generated.

A new binary vector with two reporter genes simplifies plant transformation. It aids in identifying transgenic lines, estimating gene copies, and confirming homozygous lines efficiently.

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Area of Science:

  • Molecular Biology
  • Plant Biotechnology
  • Genetics

Background:

  • Optimizing plant transformation systems is crucial for genetic research and crop improvement.
  • Efficient screening methods are needed to identify and validate transgenic plants.

Purpose of the Study:

  • To develop a novel binary vector for plant transformation.
  • To facilitate the screening and characterization of transgenic plant lines.

Main Methods:

  • Construction of a binary vector containing two distinct reporter gene cassettes.
  • Application of the vector in plant transformation experiments.

Main Results:

  • The developed vector enables the use of one reporter gene for confirming transgenic status.
  • The second reporter gene allows for the estimation of transgene locus number and zygosity determination.
  • This facilitates simple, efficient, and economical screening for homozygous single-insert lines and azygous controls.

Conclusions:

  • The novel binary vector is a valuable tool for optimizing plant transformation systems.
  • It streamlines the process of identifying and characterizing transgenic plants, particularly homozygous lines.