Controlled in vitro growth of cell microtubes: towards the realisation of artificial microvessels

The aim of this study is to realise in vitro microtubular structures for Tissue Engineering applications. The strategy adopted consists in the use of cylindrical polymeric scaffolds, on which connective tissue cells (murine fibroblasts) can adhere and proliferate. We supply stereotropic and chemical stimuli which induce the cells to cover the whole cylindrical structure and to produce an extra-cellular matrix (ECM) which acts as an adhesive for cell aggregation and assembly. Stereotropic stimuli were provided using purpose designed seeding and proliferation chambers whilst the addition of ascorbic acid to the culture medium furnished chemical stimuli. To obtain cylindrical cellular structures two approaches are considered: the first one consists in the use of biodegradable scaffolds, the second one consists in the use of non-biodegradable scaffolds which can subsequently be withdrawn. The results show that a 14-microm thick cell and ECM coating grows over nylon strands, and can also be coerced to form an effective connection system with external tubing.