Related Experiment Video

Updated: Jan 23, 2026

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

1.2K

Characterization of Staphylococcus aureus strains in a rabbit model of osseointegrated pin infections

Dustin Williams1, Roy Bloebaum, Cathy A Petti

  • 1Associated Regional and University Pathologists Laboratories, ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA. dustin.williams@aruplab.com

Journal of Biomedical Materials Research. Part A
|August 11, 2007
PubMed

Insights

This study investigated Staphylococcus aureus surgical site infections in rabbits. Results showed that the rabbits' native Staphylococcus aureus, not the applied strain, caused the infection, establishing a new rabbit model for research.

Area of Science:

  • Veterinary Medicine
  • Microbiology
  • Surgical Research

Background:

  • Staphylococcus aureus is a frequent cause of surgical site infections.
  • S. aureus can be a native or exogenous pathogen in humans and rabbits.
  • Osseointegrated implants are susceptible to bacterial colonization and infection.

Purpose of the Study:

  • To develop and validate a rabbit model for studying Staphylococcus aureus osseointegrated pin infections.
  • To investigate the source of S. aureus infection in a simulated osseointegrated pin model.
  • To differentiate between exogenous and endogenous S. aureus strains in experimental infections.

Main Methods:

  • Exogenous Staphylococcus aureus ATCC 49230 was applied weekly to osseointegrated pin sites in 20 rabbits.
  • Infected rabbits were monitored for clinical signs, leading to euthanasia and necropsy.
  • Bacterial cultures and pulsed-field gel electrophoresis (PFGE) were used to identify and differentiate S. aureus strains.

Main Results:

  • S. aureus was the predominant organism cultured from infected sites.
  • Pulsed-field gel electrophoresis (PFGE) revealed distinct genetic profiles between the applied and isolated S. aureus strains.
  • The infecting S. aureus strain was identified as native to the rabbits, not the experimentally applied exogenous strain.

Conclusions:

  • The study established a novel rabbit model for S. aureus osseointegrated pin infections.
  • The findings indicate that endogenous S. aureus strains are a significant factor in experimental infections.
  • This model can advance the understanding of S. aureus pathogenesis in osseointegrated implant-related infections.

More Related Videos

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection09:15

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection

Published on: February 28, 2019

13.2K
Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA12:18

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

Published on: February 9, 2011

23.6K

Related Concept Videos

Euler's Formula for Pin-Ended Columns01:21

Euler's Formula for Pin-Ended Columns

In structural engineering, the stability of columns under compressive axial loads is a critical consideration, described as buckling. A typical example involves a column PQ, which is pin-connected at both ends and subjected to a centric axial load F applied at one end, with a reaction force of F' = -F at the other end. Here, it is crucial to understand that when an applied load exceeds the critical load, buckling occurs as the system becomes unstable.
To calculate the critical load, envision...
696
Thermal Strain01:19

Thermal Strain

Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
2.8K
Shearing Strain01:20

Shearing Strain

The shearing strain represents a cubic element's angular change when subjected to shearing stress. This type of stress can transform a cube into an oblique parallelepiped without influencing normal strains. The cubic element experiences a significant transformation when exposed solely to shearing stress. Its shape alters from a perfect cube into a rhomboid, clearly demonstrating the effect of shearing strain. The degree of this strain is considered positive if it reduces the angle between the...
1.3K
Measurements of Strain01:27

Measurements of Strain

Strain quantifies the deformation of a material under force, typically measured as normal strain, which represents the change in length when compared with the original length. Electrical strain gauges are used for enhanced accuracy. These devices consist of a conductive wire mounted on a paper backing that adheres to the material's surface. These gauges operate on the piezoresistive effect, where the wire's electrical resistance changes in response to mechanical deformation. The strain...
2.6K
Strain Energy01:13

Strain Energy

Strain energy is a fundamental concept in the field of materials science and structural engineering, describing the energy absorbed by a material or structure when it is deformed under load.
Consider a rod that is fixed at one end and subjected to an axial force at the free end. This axial force induces stress within the rod, leading to its elongation. As the axial force increases, so does the elongation of the rod, illustrating a direct relationship between the force applied and the resulting...
936
Stress-Strain Diagram01:10

Stress-Strain Diagram

A stress-strain diagram is a crucial tool that graphically displays a material's mechanical characteristics. This diagram is derived from a tensile test performed on a carefully prepared cylindrical specimen. The specimen has two gauge marks inscribed on its central part, and the distance between these marks is known as the gauge length. The cylindrical specimen is placed in a testing machine, which applies an increasing centric load. As this load grows, so does the gauge length. This...
2.3K