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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jul 13, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

GFP expression alters osteosarcoma cell biology.

Crispin R Dass¹, Peter F Choong

¹Department of Orthopaedics, University of Melbourne/St. Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia. crispin.dass@svhm.org.au

DNA and Cell Biology

|August 11, 2007

Summary

Green fluorescent protein (GFP) expression, used for cell tracking, may increase osteosarcoma tumor aggression. Researchers caution its use in animal models due to observed effects on bone markers and cell biology.

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Area of Science:

Oncology
Molecular Biology
Biotechnology

Background:

Green fluorescent protein (GFP) is a common tool for in vivo cell tracking.
Osteosarcoma research often utilizes animal models for studying tumor progression.

Purpose of the Study:

To investigate the impact of GFP transfection on human osteosarcoma cell lines.
To assess potential alterations in tumor behavior and cellular physiology post-transfection.

Main Methods:

Transfection of three human osteosarcoma cell lines with GFP.
Analysis of tissue marker expression and cellular physiology in vitro.
Evaluation of in vivo tumor aggression in transfected cell lines.

Main Results:

GFP transfection led to increased tumor aggression in one osteosarcoma cell line.
Altered expression of bone markers and osteoblast-like cell biology was observed in vitro.
The observed in vivo effects correlated with in vitro cellular changes.

Conclusions:

GFP expression may influence osteosarcoma tumor behavior and cellular characteristics.
Caution is advised when using GFP for cell tracking in osteosarcoma animal models.
Further research is needed to understand the mechanisms behind GFP-mediated effects.