Related Experiment Video
Updated: Jul 13, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
In silico analysis of p53 using the p53 knowledgebase: mutations, polymorphisms, microRNAs and pathways
Yuchen Yang1, Erwin Tantoso, Gek Huey Chua
1Institute of Molecular and Cell Biology, Proteos, Singapore.
In Silico Biology
|August 11, 2007
Summary
The p53 tumor suppressor gene is crucial for cancer prevention. This study created a p53 knowledgebase, revealing new insights into cancer mutations and gene regulation.
Area of Science:
- Molecular Biology
- Bioinformatics
- Genetics
Background:
- The p53 gene is a critical tumor suppressor with extensive research.
- A comprehensive knowledgebase is needed to consolidate p53 information for researchers.
Purpose of the Study:
- To develop a p53 knowledgebase for experimental design and data analysis.
- To perform bioinformatics analyses for novel discoveries regarding p53.
Main Methods:
- Curated data on p53 mutations, transcription factors, targets, and SNPs.
- Bioinformatics analysis of curated data.
- Assessment of tagSNP selection strategies using HapMap and NIEHS data.
Main Results:
- Identified over-represented point missense mutations in cancers lacking functional studies.
- NIEHS data is superior for tagSNP selection in p53 association studies.
- Discovered potential microRNA regulation of p53 transcription factors.
- Differentiated p53 transcriptional controls in cell cycle and apoptosis pathways.
Conclusions:
- The p53 knowledgebase facilitates research and discovery.
- Bioinformatics analysis yields significant new findings on p53.
- Strategic SNP selection enhances future genetic association studies.
Related Concept Videos
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...