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Related Experiment Video

Updated: Jul 13, 2026

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
11:38

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

Published on: July 22, 2014

MR diffusion changes correlate with ultra-structurally defined axonal degeneration in murine optic nerve.

Qizhu Wu1, Helmut Butzkueven, Melissa Gresle

  • 1Howard Florey Institute, University of Melbourne, VIC 3010, Australia.

Neuroimage
|August 11, 2007
PubMed
Summary

Diffusion Tensor Imaging (DTI) reveals reduced parallel diffusivity in optic neuritis, correlating with axonal loss. This MRI technique shows promise for tracking neuroprotection in preclinical studies.

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Area of Science:

  • Neuroimaging
  • Neuroscience
  • Biomedical Engineering

Background:

  • Diffusion weighted imaging (DWI) and diffusion tensor imaging (DTI) are key for studying central nervous system (CNS) white matter.
  • Changes in diffusivity correlate with axonal pathology and demyelination, but underlying ultra-structural changes require elucidation.

Purpose of the Study:

  • To investigate the relationship between directional diffusivities and ultra-structural properties in the mouse optic nerve.
  • To assess the feasibility of MRI for detecting axonal degeneration in a mouse model of optic neuritis (ON).

Main Methods:

  • Utilized diffusion MRI (DWI/DTI) on healthy mice and mice with induced optic neuritis (ON).
  • Examined optic nerves for inflammatory changes, axonal pathology, and correlated diffusivity measurements with ultra-structural properties.

Main Results:

  • Optic neuritis induced a significant 23% reduction in parallel diffusivity (P<10(-5)) without altering perpendicular diffusivity.
  • Reduced parallel diffusivity strongly correlated with decreased total axolemmal cross-sectional area (r=0.86, P<0.001).

Conclusions:

  • Reduced parallel diffusivity in the optic nerve is a quantitative marker of axonal degeneration.
  • MRI assessment of axonal degeneration in murine ON is feasible and valuable for preclinical neuroprotection trials.