Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gradient Optical Response of Au Ellipse Nanopillar Array via Anisotropic Pillar Heights: A Highly Tunable Plasmonic Material toward Biosensing Applications.

ACS nanoscience Au·2026
Same author

Surface Modification of Screen-Printed Carbon Electrode through Oxygen Plasma to Enhance Biosensor Sensitivity.

Biosensors·2024
Same author

Wireless electrochemiluminescent biosensors: Powering innovation with smartphone technology.

Biosensors & bioelectronics·2024
Same author

Miniaturization of CRISPR/Cas12-Based DNA Sensor Array by Non-Contact Printing.

Micromachines·2024
Same author

Solid-Phase Collateral Cleavage System Based on CRISPR/Cas12 and Its Application toward Facile One-Pot Multiplex Double-Stranded DNA Detection.

Bioconjugate chemistry·2023
Same author

Fully Transparent, Ultrathin Flexible Organic Electrochemical Transistors with Additive Integration for Bioelectronic Applications.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2022

Related Experiment Video

Updated: Jul 13, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

A single-bead analysis on a disk-shaped microfluidic device using an antigen-immobilized bead.

Hidenori Nagai1, Yuka Narita, Miyuki Ohtaki

  • 1Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Midorigaoka, Ikeda, Osaka, Japan.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|August 11, 2007
PubMed
Summary

A novel lab-on-a-disk device enables mental stress evaluation by measuring secretory immunoglobulin A (sIgA) using centrifugal force. This automated enzyme immunoassay (EIA) offers a miniaturized alternative to traditional methods.

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jul 13, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Mental stress evaluation is crucial for health monitoring.
  • Secretory immunoglobulin A (sIgA) is a potential biomarker for mental stress.
  • Conventional enzyme immunoassays (EIA) can be time-consuming and require significant sample volumes.

Purpose of the Study:

  • To develop a disk-shaped microfluidic device (lab-on-a-Disk) for automated mental stress evaluation.
  • To measure sIgA levels using heterogeneous enzyme immunoassay (EIA) on the developed platform.
  • To demonstrate the feasibility of centrifugal force for microfluidic control in the device.

Main Methods:

  • A disk-shaped microfluidic device was designed and fabricated.
  • Centrifugal force was utilized to control microfluidic operations, including sample manipulation and sequential reactions.
  • A heterogeneous enzyme immunoassay (EIA) was performed on the device using sIgA immobilized on glass beads.
  • The relationship between rotational speed, channel profile, and chamber position was analyzed for optimal microfluidic control.

Main Results:

  • The lab-on-a-Disk successfully integrated microfluidic control and EIA for sIgA measurement.
  • Centrifugal force enabled precise manipulation of sample solutions through microchannels and reservoirs.
  • The device demonstrated potential for miniaturization and automation of EIA processes.
  • The assay format involved a competitive antigen-antibody reaction using immobilized sIgA.

Conclusions:

  • The developed lab-on-a-Disk is a promising platform for the miniaturized and automated measurement of sIgA.
  • This device offers a potential alternative to conventional EIA for mental stress evaluation.
  • The study highlights the effectiveness of centrifugal microfluidics for complex bioassays.