Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The FOXA transcription factors in allergic airway disease and asthma.

Frontiers in allergy·2026
Same author

Organ-specific features of human kidney lymphatics are disrupted in chronic transplant rejection.

The Journal of clinical investigation·2025
Same author

Distinctive T-cell receptor repertoire in paediatric inflammatory multisystem syndrome temporally associated with coronavirus disease 2019/multisystem inflammatory syndrome in children patients: possible thymus involvement.

Clinical and experimental immunology·2025
Same author

Reduction of elevated Gli3 does not alter the progression of autosomal recessive polycystic kidney disease.

Physiological reports·2025
Same author

Distinct T-cell receptor (TCR) gene segment usage and MHC-restriction between foetal and adult thymus.

eLife·2024
Same author

A multidimensional toolkit for elucidating temporal trajectories in cell development in vivo.

Development (Cambridge, England)·2024

Related Experiment Video

Updated: Jul 13, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

Sonic hedgehog signalling in T-cell development and activation.

Tessa Crompton1, Susan V Outram, Ariadne L Hager-Theodorides

  • 1Immunobiology Unit, University College London Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK. t.crompton@ich.ucl.ac.uk

Nature Reviews. Immunology
|August 11, 2007
PubMed
Summary

The thymic epithelium regulates T cell development by secreting sonic hedgehog (SHH). This signaling pathway is crucial for thymocyte differentiation, proliferation, and T-cell receptor selection in the thymus and peripheral T cell activation.

More Related Videos

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
07:46

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury

Published on: June 29, 2015

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

Related Experiment Videos

Last Updated: Jul 13, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
07:46

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury

Published on: June 29, 2015

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Signaling

Background:

  • Mature T cell production in the thymus depends on signals from the thymic epithelium.
  • The thymic epithelium plays a critical role in guiding T cell differentiation and selection.

Purpose of the Study:

  • To review the role of sonic hedgehog (SHH) secreted by the thymic epithelium in T cell development.
  • To explore the impact of SHH signaling on thymocyte differentiation, proliferation, and T cell repertoire selection.
  • To examine the influence of hedgehog signaling on peripheral T cell activation.

Main Methods:

  • Review of recent experimental findings on SHH signaling in T cell development.
  • Analysis of the literature on hedgehog pathway involvement in thymic and peripheral T cell biology.

Main Results:

  • Sonic hedgehog (SHH) secreted by the thymic epithelium is a key regulator of T cell production.
  • SHH signaling is essential for early thymocyte progenitor differentiation and proliferation.
  • SHH influences T-cell receptor signaling during repertoire selection, including positive selection, CD4/CD8 lineage commitment, and clonal deletion.
  • Hedgehog signaling also impacts peripheral T cell activation.

Conclusions:

  • Sonic hedgehog (SHH) is a critical signaling molecule from the thymic epithelium that orchestrates T cell development and function.
  • Understanding SHH's role provides insights into T cell maturation, selection processes, and potential therapeutic targets for immune disorders.