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Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells

Takahisa Kuga1, Yuji Nakayama, Masaki Hoshino

  • 1Department of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8675, Japan.

Archives of Biochemistry and Biophysics
|August 19, 2007
PubMed

Insights

Src-family tyrosine kinases (SFKs) are crucial for mitosis. This study reveals differential activation of c-Src, c-Yes, and Lyn during M phase, mediated by Cdc2 kinase.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Src-family tyrosine kinases (SFKs) are critical regulators of cell division.
  • Understanding the specific roles and activation mechanisms of individual SFKs during mitosis remains incomplete.

Purpose of the Study:

  • To investigate the differential activation of endogenous c-Src, c-Yes, and Lyn during M phase.
  • To elucidate the role of Cdc2 kinase in the mitotic activation of these SFKs.

Main Methods:

  • Analysis of endogenous c-Src, c-Yes, and Lyn activation in HeLa cells during mitosis.
  • Electrophoretic mobility shift assays to detect post-translational modifications.
  • Inhibition studies using Cdc2 inactivation.

Main Results:

  • c-Src, c-Yes, and Lyn exhibited distinct activation levels during M phase.
  • Cdc2 inactivation inhibited the activation of all three SFKs.
  • Mitotic c-Src and c-Yes showed mobility retardation due to Cdc2-mediated phosphorylation, unlike Lyn.
  • The retarded mobility form of c-Yes displayed higher activation than its normal mobility form.

Conclusions:

  • Endogenous c-Src, c-Yes, and Lyn are differentially activated during M phase.
  • Cdc2 kinase plays a key role in mediating the mitotic activation and phosphorylation of c-Src and c-Yes.

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