Related Experiment Video
Updated: Jul 13, 2026

Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
Published on: May 5, 2021
High glucose enhances MMP-2 production in adventitial fibroblasts via Akt1-dependent NF-kappaB pathway
Seung Jin Lee1, Sun Sik Bae, Koan Hoi Kim
1Department of Pharmacology, BK21 Medical Science Education Center, College of Medicine, Pusan National University, 10 Ami-Dong 1-Ga, Seo-Gu, Busan 602-739, Republic of Korea.
Abstract:
To understand the role of adventitial fibroblasts (AF) in diabetic vascular diseases, the importance of high glucose (HG, 25mM) on matrix metalloproteinase-2 (MMP-2) production in AF was determined. HG enhanced mRNA, protein and gelatinolytic activity of MMP-2. The enhanced MMP-2 activity was significantly attenuated not only by a PI3K inhibitor but also by an Akt inhibitor. These HG-induced MMP-2 responses were markedly reduced in Akt1-deficient (1KO) cells. The diminished HG-induced MMP-2 responses were completely restored by re-expression of Akt1. Both the reporter activity and electrophoretic mobility shift assay for activator protein-1 and nuclear factor-kappa B (NF-kappaB) were enhanced by HG, but NF-kappaB were not increased in 1KO cells. Furthermore, HG-induced MMP-2 responses were markedly suppressed by NF-kappaB decoy oligodeoxynucleotides. Based on these results, it is suggested that HG augments MMP-2 production via PI3K/Akt1/NF-kappaB pathway.
Related Concept Videos
cAMP-dependent Protein Kinase Pathways
PI3K/mTOR/AKT Signaling Pathway
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Intracellular Signaling Affects Focal Adhesions
Some...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are co-secreted in...
Regulation of Angiogenesis and Blood Supply