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Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
COP Coated Vesicles00:59

COP Coated Vesicles

Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview

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Related Experiment Video

Updated: Jul 13, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

The interplay between clathrin-coated vesicles and cell signalling.

Ian G Mills1

  • 1Cancer Research UK, Cambridge Research Institute, Robinson Way, Cambridge CB2 ORE, UK. ian.mills@cancer.org.uk

Seminars in Cell & Developmental Biology
|August 19, 2007
PubMed
Summary

Cell surface internalization of proteins and lipids uses clathrin-mediated endocytosis. Signaling events regulate protein-lipid interactions, controlling clathrin coat assembly and disassembly during membrane trafficking.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell surface internalization of proteins and lipids is crucial for cellular functions.
  • This process occurs constitutively and is regulated by signaling events.
  • Clathrin-mediated endocytosis is a primary pathway for cargo uptake, especially in response to signaling.

Purpose of the Study:

  • To investigate the integration of signaling cascades with vesicle coat dynamics.
  • To understand the role of protein-lipid and protein-protein interactions in cargo targeting and clathrin coat assembly.
  • To explore the mechanisms underlying the assembly and disassembly of clathrin coats in response to cellular signals.

Main Methods:

  • Analysis of protein-lipid and protein-protein interactions at the cell membrane.
  • Investigating clathrin-coated vesicle formation and dynamics.
  • Studying the regulation of endocytic pathways by signaling events.

Main Results:

  • Protein-lipid and protein-protein interactions are key regulators of cargo targeting to membrane compartments.
  • These interactions also control the assembly of clathrin coats.
  • Signaling cascades are integrated with the processes of clathrin coat assembly and disassembly.

Conclusions:

  • Signaling events dynamically regulate clathrin-mediated endocytosis.
  • The interplay between signaling and vesicle coat dynamics is essential for membrane trafficking.
  • Further research into this integration provides insights into cellular regulation and disease mechanisms.