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Updated: Jul 13, 2026

Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
Bone voyage: an expedition into the molecular and cellular parameters affecting bone graft fate
J A Helms1, R R Amasha, P Leucht
1Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford Medical School, Stanford, CA 94305, USA. jhelms@stanford.edu
Abstract:
The demand for bone grafts in orthopaedic and craniofacial surgery is steadily increasing. Estimations suggest that about 500,000 are performed annually in the United States that include bone grafting as a component of the surgery, and the majority of these surgeries employ autografts. This perspective focuses on the biological events that occur during osseointegration of such bone grafts. Here, three key factors of graft osseointegration--the embryonic origin, the inclusion of skeletal progenitor cells, and the integrity of the recipient site--are discussed. Altogether, they form the foundation for survival of the bone graft and eventually for a positive clinical outcome of the procedure.
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