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Molecular cloning and expression analysis of pig CD81
Kyu-Won Cho1, Sang Joon Kim, Chung-Gyu Park
1Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Veterinary Immunology and Immunopathology
|August 19, 2007
Summary
Researchers cloned pig CD81 (target of antiproliferative antibody 1) cDNA, revealing its sequence and broad tissue expression. This tetraspanin protein is widely distributed, suggesting diverse immune functions in pigs.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- CD81 (target of antiproliferative antibody 1) is a tetraspanin protein crucial for B and T cell activation.
- Understanding CD81's role in swine is vital for immunology and disease research.
Purpose of the Study:
- To clone and characterize the pig Cd81 cDNA sequence.
- To investigate the expression patterns of pig CD81 in various tissues.
- To confirm the recognition of pig CD81 by human antibodies.
Main Methods:
- Cloning of pig Cd81 cDNA using RT-PCR and RACE-PCR.
- Sequence analysis and comparison with mammalian orthologs.
- RT-PCR for mRNA expression analysis across tissues.
- Flow cytometry and immunohistochemistry for protein expression and localization.
Main Results:
- Complete pig Cd81 cDNA sequence obtained (711 bp, 236 amino acids).
- High sequence identity of pig CD81 with human (90.30%) and cattle (92.26%).
- Pig CD81 mRNA detected in both lymphoid and non-lymphoid tissues.
- Human CD81 antibody successfully recognized pig CD81 on cell surfaces and in spleen tissue.
Conclusions:
- Pig CD81 is a conserved protein with broad tissue distribution.
- CD81 likely plays diverse roles in various pig tissues, including immune functions.
- Further research will explore CD81's role in pig infectious diseases.
