Related Experiment Video
Updated: Jul 13, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Sequence analysis of the Staphylococcus aureus srrAB loci reveals that truncation of srrA affects growth and
Alexa A Pragman1, Lisa Herron-Olson, Laura C Case
1Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Abstract:
The SrrAB system regulates metabolism and virulence factors in Staphylococcus aureus. We sequenced the srrAB loci of 21 isolates and performed a phylogenetic analysis. Vaginal and bovine isolates clustered together, while skin isolates were genetically diverse. Few nucleotide polymorphisms were observed, and most were synonymous. Two strains (N2 and N19) with N-terminal truncations in SrrA displayed defects in growth and abnormally upregulated virulence factor expression under low-oxygen conditions.
Related Concept Videos
Clinical Significance of Antibiotic Resistance
Staphylococcal Skin Infections
Mechanism of Antibiotic Resistance in MRSA
Regulation of Bacterial Virulence
Determinants of Bacterial Pathogenicity and Virulence
Gene Regulation in Microbial Communities: Quorum Sensing
