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Updated: Jul 13, 2026
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Published on: July 28, 2023
[Experimental study on fas gene death domain mutations in keloid pedigrees]
Xiaojun Liu1, Jianhua Gao, Xuelei Li
1lxjun_ok@126.com
Objective:
To detect gene mutations of Fas gene death domain (exons 79) in 2 Chinese keloid pedigrees and to investigate the significance of Fa gene mutations in the keloid formation.
Methods:
The samples were selected from keloid pedigrees A and B in 2005. The polymerase chain reaction and DNA sequencing analysis technique were used to detect the sequence of exons 7-9 of Fas gene from keloid tissues of 2 male patients in pedigree A, their peripheral vein blood and their surrounding normal skin served as their own contrast, their spouses' peripheral vein blood served as normal contrast, the peripheral vein blood of 2 patients in pedigree B served as a contrast between different keloid pedigrees.
Results:
No gene mutations and single nucleotide polymorphism in Fas gene exons 7, 8 were found in all samples from pedigrees A and B. But point mutations and single nucleotide polymorphism in Fas gene exon 9 were identified in 11 bp and 53 bp in 2 keloid tissue samples from Chinese keloid pedigree A.
Conclusion:
Fas gene point mutations maybe indicate some relations in Fas protein function and keloid formation.
