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Updated: Jul 13, 2026

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
Effect of wt-P53 protein on telomerase activity in keloid fibroblasts
Zhibo Xiao1, Lijun Hao, Lihong Ren
1Cosmetic and Plastic Center, the 2nd Affiliated Hospital, Harbin Medical University, Harbin Heilongjiang, 150086, PR China. xiaozhibodoctor@yahoo.com.cn
Objective:
To evaluated the role of wt-P53 protein in telomerase regulation in keloid fibroblasts (KFBs).
Methods:
The fibroblasts were derived from human keloid tissue which was proved by pathological diagnosis. KFBs were divided into 2 groups, the transfection group and the untransfection group. wt-p53 gene was transfected into the fibroblasts by adenovirus vectors in the transfection group. The KFBs untransfected with wt-p53 gene served as control (untransfection group). After 48 hours of transfection, the expression of wt-P53 protein was analyzed by both Western blotting and immunofluorescence method, respectively. The telomerase activity was evaluated by TRAP-ELISA after 1-7 days of transfection.
Results:
All the KFBs from 2 groups expressed wt-P53 protein. But the expression level of wt-P53 protein in the transfection group was significantly higher than that in the untransfection group. At the same time of high expression of wt-P53 protein, the telomerase activity of KFBs in transfection group was significantly lower than that in the untransfection group (P < 0.05).
Conclusion:
High level expression of wt-P53 protein can transiently inhibit the telomerase activity of KFBs.
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