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Lipoprotein (a) and apolipoprotein (a) phenotypes in healthy Macedonian children

K Tosheska1, D Labudovic, S Alabakovska

  • 1Department of Medical and Experimental Biochemistry, Medical Faculty, Skopje, Republic of Macedonia. tosheskatrajkovska@gmail.com

Bratislavske Lekarske Listy
|August 19, 2007
PubMed

Insights

Genetic factors determine lipoprotein(a) [Lp(a)] levels, a risk for atherosclerosis. This study analyzed Lp(a) levels and apolipoprotein(a) [apo(a)] phenotypes in healthy Macedonian children, finding an inverse relationship between apo(a) size and Lp(a) concentration.

Area of Science:

  • Cardiovascular Genetics
  • Biochemistry
  • Pediatric Health

Background:

  • Plasma lipoprotein(a) [Lp(a)] levels are primarily determined by genetic variations in the apolipoprotein(a) [apo(a)] gene.
  • Elevated Lp(a) levels are a significant risk factor for premature atherosclerosis.
  • The relationship between apo(a) phenotypes and disease development is not well understood.

Purpose of the Study:

  • To determine lipoprotein(a) [Lp(a)] levels and apolipoprotein(a) [apo(a)] isoforms (phenotypes) in healthy Macedonian children.
  • To investigate the association between apo(a) phenotypes and plasma Lp(a) concentrations in a pediatric cohort.

Main Methods:

  • Utilized 3-15% gradient SDS-PAGE for the separation and classification of apo(a) isoforms.
  • Phenotypes were categorized into single-band (homozygotic), double-band (heterozygotic), and null phenotypes based on electrophoretic mobility.
  • Plasma Lp(a) levels were quantified and analyzed in relation to apo(a) phenotypes.

Main Results:

  • Single-band apo(a) phenotypes were more frequent (64%) than double-band (32%) and null phenotypes (4%).
  • The S4 phenotype was the most common (42.65%).
  • A significant inverse correlation was observed between apo(a) molecular weight and plasma Lp(a) concentration (r = -0.4257).

Conclusions:

  • Lp(a) levels and apo(a) phenotypes in children can be determined using SDS-PAGE.
  • Understanding these associations may aid in the prevention and reduction of atherosclerotic risk in pediatric populations.
  • Further research into apo(a) phenotypes and Lp(a) levels in children is warranted.
Abstract

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