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DNA of some anaerobic rumen fungi: G + C content determination
G Billon-Grand1, J B Fiol, A Breton
1ICBMC, Section Levures, Université Lyon 1, Villeurbanne, France.
FEMS Microbiology Letters
|August 15, 1991
Abstract:
The nuclear DNAs from five species of anaerobic rumen fungi have been isolated and purified by means of two extraction methods (with and without 8 M urea). Their G + C contents have been characterized by the thermal denaturation procedure of Marmur and Doty. As has already been shown in Neocallimastix frontalis, the results obtained by the two techniques demonstrated a very low G + C content (less than 20%) and the constant presence of satellite DNA.