Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
PCR01:32

PCR

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Sheathia shaoguanensis</i> sp. nov. (Batrachospermales, Rhodophyta), a new freshwater red algal species from South China.

PhytoKeysĀ·2026
Same author

[<i>Tongyuan</i> acupuncture for postherpetic neuralgia: a randomized controlled trial].

Zhongguo zhen jiu = Chinese acupuncture & moxibustionĀ·2026
Same author

Aging Enhances Serotonergic Signaling via 5-HT<sub>7</sub> Receptors Underlying Mechanical Alloknesis.

Aging cellĀ·2026
Same author

Multi-omics analysis reveals mechanisms of Qingying granules in treating porcine warm disease: dosage optimization and systems biology insights.

BMC veterinary researchĀ·2026
Same author

Amino-acids-mTORC1-driven DDA1 phosphorylation promotes DNA repair and glioblastoma progression.

Cell communication and signaling : CCSĀ·2026
Same author

Structural insights into the specificity of adaptor proteins Dok5 and Dok6 Docking to Trk receptors.

Cell communication and signaling : CCSĀ·2026

Related Experiment Video

Updated: Jul 13, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

PCR-based procedures to isolate insertion sites of DNA elements.

Bin Yin1, David A Largaespada

  • 1University of Minnesota, Minneapolis, MN, USA. binxx002@umn.edu

Biotechniques
|August 19, 2007
PubMed
Summary

New PCR methods, SplinkTA-PCR (STA-PCR) and SplinkBlunt-PCR, efficiently isolate retroviral insertion sites. Combining these techniques maximizes cancer gene discovery through saturation analysis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Retroviral insertional mutagenesis is a key tool for identifying genes involved in cancer.
  • Accurate identification of proviral insertion sites is crucial for this approach.
  • Existing methods for insertion site identification require improvement for comprehensive cancer gene discovery.

Purpose of the Study:

  • To develop improved methods for isolating retroviral insertion sites.
  • To enhance the efficiency of saturation analysis for cancer gene discovery.
  • To provide reliable and scalable protocols for identifying integrated DNA elements.

Main Methods:

  • Development of two novel ligation-mediated PCR variants: SplinkTA-PCR (STA-PCR) and SplinkBlunt-PCR.
  • Application of these methods for efficient isolation of insertion sites in retrovirus-induced leukemia models.

More Related Videos

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Linear Amplification Mediated PCR &#8211; Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

Related Experiment Videos

Last Updated: Jul 13, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Linear Amplification Mediated PCR &#8211; Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

  • Evaluation of the complementary nature and combined efficiency of the developed protocols.

    • Main Results:

    • STA-PCR and SplinkBlunt-PCR protocols were successfully developed and demonstrated.
    • The two methods are complementary, with their combination yielding maximal cloning efficiency.
    • The protocols are effective for large-scale cloning of proviral insertion sites and other integrated DNA elements.

    Conclusions:

    • The developed STA-PCR and SplinkBlunt-PCR methods offer an improvement for isolating retroviral insertion sites.
    • Combining these complementary protocols enhances efficiency for cancer gene discovery via saturation analysis.
    • These protocols are versatile, reliable, and suitable for large-scale applications in genetic research and drug resistance studies.