Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An investigation into 53BP1 complex formation.

Kevin C Roche1, Noel F Lowndes

  • 1National University of Ireland Galway, Department of Biochemistry. kevin.roche@nuigalway.ie

Advances in Experimental Medicine and Biology
|August 19, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Regulation of the DNA Damage Response and Gene Expression by the Dot1L Histone Methyltransferase and the 53Bp1 Tumour Suppressor.

PloS one·2024
Same author

DDX17 is required for efficient DSB repair at DNA:RNA hybrid deficient loci.

Nucleic acids research·2022
Same author

A function for ataxia telangiectasia and Rad3-related (ATR) kinase in cytokinetic abscission.

iScience·2022
Same author

Immediate-Early, Early, and Late Responses to DNA Double Stranded Breaks.

Frontiers in genetics·2022
Same author

Novel Pt(IV) Prodrugs Displaying Antimitochondrial Effects.

Molecular pharmaceutics·2020
Same author

Analysis of novel missense ATR mutations reveals new splicing defects underlying Seckel syndrome.

Human mutation·2018

This study investigates 53BP1 protein complex formation and oligomerization. The findings indicate that 53BP1 oligomerization does not require disulfide bridges, offering insights into DNA damage response mechanisms.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Cellular proliferation control is crucial for preventing cell death and cancer.
  • The DNA damage checkpoint pathway monitors genome integrity.
  • 53BP1 protein interacts with the tumor suppressor p53 and is involved in DNA damage response.

Purpose of the Study:

  • To investigate 53BP1 complex formation.
  • To analyze 53BP1 oligomerization.
  • To determine the role of disulfide bridges in 53BP1 oligomerization.

Main Methods:

  • Protein interaction studies
  • Oligomerization assays
  • Analysis of disulfide bridge dependence

Main Results:

Related Experiment Videos

  • 53BP1 forms complexes and oligomers.
  • 53BP1 oligomerization is independent of disulfide bridges.

Conclusions:

  • Disulfide bridges are not essential for 53BP1 oligomerization.
  • Further research is needed to fully understand 53BP1's roles in DNA metabolism and checkpoint response.