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Complete nucleotide sequence of the human RCC1 gene involved in coupling between DNA replication and mitosis
N Furuno1, K Nakagawa, U Eguchi
1Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
Abstract:
Total genomic DNA of the human RCC1 gene was isolated from HeLa DNA and its complete nucleotide sequence (34,641 bp) was determined by the shotgun sequencing method. The exon-intron junctions were precisely assigned to this sequence by comparing the nucleotide sequence of RCC1 genomic DNA with that of its cDNA. The RCC1 gene was found to have 14 exons, 8 of which (starting from the seventh one) coded the seven repeated sequences of RCC1 protein. A single exon corresponded roughly to each repeat of the RCC1 protein except for the middle one, indicating that the RCC1 gene was generated through amplification of a primordial exon. Primer extension analysis revealed the presence of an internal promoter.