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Requirements for relative intensity correction of Raman spectra obtained by column-summing charge-coupled device

Wilbur S Hurst¹, Steven J Choquette, Edgar S Etz

¹Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA. wilbur.hurst@nist.gov

Applied Spectroscopy

|August 21, 2007

Summary

Accurate Raman spectra intensity correction requires careful consideration of charge-coupled device (CCD) detector operation. Summing CCD pixel rows can introduce spectral distortions if the light source

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Area of Science:

Spectroscopy
Analytical Chemistry
Instrumentation

Background:

Relative intensity correction is crucial for accurate Raman spectra analysis.
Raman spectrometers often utilize two-dimensional charge-coupled device (CCD) array detectors.
CCD detectors can operate in different modes, including summing pixel rows.

Purpose of the Study:

To investigate the impact of CCD row summing on Raman spectra intensity correction.
To identify restrictions on light source spatial intensity profiles for accurate correction.
To quantify potential spectral distortions introduced by the row summing method.

Main Methods:

Development of numerical expressions to define restrictions for accurate intensity correction.
Modeling of different cases to estimate the magnitude of spectral distortions.
Analysis of inherent pixel quantum efficiency variations in CCD detectors.
Evaluation of spectral effects caused by localized pixel quantum efficiency changes.

Main Results:

Operation mode where CCD rows are summed imposes specific spatial intensity profile requirements on the calibration light source.
These requirements can also extend to the spatial intensity profile of the measured Raman signal.
Significant spectral distortions can arise when these restrictions are not met, particularly with non-uniform pixel quantum efficiency.

Conclusions:

Proper selection and characterization of the irradiance source are critical for accurate Raman spectra.
Understanding CCD detector spatial response and pixel quantum efficiency is essential to avoid spectral artifacts.
The study provides a framework for deriving and applying constraints to ensure reliable intensity correction.