Related Experiment Video
Updated: Jul 13, 2026

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
Published on: August 31, 2018
A novel nacre protein N19 in the pearl oyster Pinctada fucata
Masato Yano1, Kouhei Nagai1, Koichi Morimoto2
1Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, Japan Science and Technology Agency, Japan.
Abstract:
A novel 19kDa protein, which was named N19, was isolated from the nacreous layer of the pearl oyster Pinctada fucata. N19 is one of predominant proteins found in the water-insoluble fraction of the nacreous layer. MALDI-TOF/TOF analysis indicated that the three trypsin-digested peptides (791.45, 824.42, and 1118.65m/z) corresponded to the amino acid sequences predicted from a cDNA isolated from a mantle cDNA library of P. fucata. Northern blot analysis revealed that the N19 mRNA was a little more abundant in the pallial region than the edge region, in the mantle. In CaCO(3) precipitation assay, the recombinant N19 protein inhibited the crystallization of CaCO(3). These results indicate that N19 is localized in the nacre and plays a negative regulatory role in calcification in the pearl oyster.
More Related Videos
07:53Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods
Published on: September 5, 2018
12:14A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Related Concept Videos
GPI Anchoring of Proteins in the ER Membrane
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
Nuclear Protein Sorting
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Regulation of Nuclear Protein Sorting
Accessory Structures of the Skin: Nails
The main components of a nail include the following.
Nail Plate: The nail plate is the visible portion of the nail that extends beyond the fingertips or toes. It is a hard, translucent...
Nuclear Export of mRNA
Structure of Porins