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Published on: June 14, 2017
Biomaterial-mediated retroviral gene transfer using self-assembled monolayers
Charles A Gersbach1, Sean R Coyer, Joseph M Le Doux
1Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, Atlanta, GA 30332, USA.
Biomaterials
|August 19, 2007
Summary
Biomaterials can immobilize retroviral particles for efficient gene delivery. Amine-terminated surfaces achieved over 90% gene transfer, advancing gene therapy strategies.
Area of Science:
- Biomaterials Science
- Gene Therapy
- Cell Biology
Background:
- Biomaterial-mediated gene delivery offers an alternative to viral vectors.
- Current strategies often focus on transient gene expression.
- Retroviral particle immobilization on biomaterials is less explored.
Purpose of the Study:
- To characterize biomaterial properties for retroviral particle immobilization.
- To assess gene transfer efficiency at the cell-material interface.
- To optimize biomaterial-mediated gene delivery.
Main Methods:
- Utilized self-assembled monolayers (SAMs) of alkanethiols on gold surfaces.
- Functionalized SAMs with different chemical terminations (NH2, COOH, CH3).
- Quantified gene transfer efficiency via retroviral transduction of mammalian cells.
Main Results:
- NH2-terminated SAMs achieved >90% gene transfer.
- COOH-functionalized SAMs yielded ~50% gene transfer.
- CH3-terminated SAMs showed no detectable gene transfer, similar to controls.
Conclusions:
- Amine-terminated SAMs are highly effective for biomaterial-mediated retroviral gene delivery.
- This approach offers a promising platform for safe and efficient gene therapy.
- Optimized biomaterial surfaces enhance gene transfer efficiency at the cell-material interface.
