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Updated: Jul 13, 2026
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
Published on: November 3, 2009
Enhanced concentration and isolation of Cyclospora cayetanensis oocysts from human fecal samples
Diana K Riner1, Andrew S Mullin, Sasha Y Lucas
1Pegasus Technical Services Inc., 46 E. Hollister St. Cincinnati, OH 45219, United States.
Abstract:
Cyclospora cayetanensis is the causative agent of cyclosporiasis, an emerging infectious disease. We present a new method for the purification of C. cayetanensis oocysts from feces using a modified detachment solution and Renocal-sucrose gradient sedimentation. This method yields oocysts free from adherent fecal debris and amenable to processing using flow cytometry.
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