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Updated: Jul 13, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

Rescuing yeast mutants with human genes.

Michael J Osborn¹, J Ross Miller

¹The Babraham Institute, Cambridge CB2 4AT, UK.

Briefings in Functional Genomics & Proteomics

|August 19, 2007

Summary

Yeast gene complementation allows researchers to identify human genes with similar functions. This powerful technique has successfully uncovered genes involved in crucial processes like apoptosis and steroid signaling.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Schizosaccharomyces pombe and Saccharomyces cerevisiae are well-established model organisms.
These yeasts have been used for decades to isolate genes from other species.
Gene isolation is often achieved through complementation of yeast mutations using heterologous cDNA libraries.

Purpose of the Study:

To highlight the utility of yeast gene complementation as a tool for functional genomics.
To demonstrate the successful application of yeast complementation in identifying human genes.
To showcase the use of yeast systems for studying conserved biological pathways like apoptosis and steroid receptor signaling.

Main Methods:

Utilizing mutations in fission yeast (Schizosaccharomyces pombe) and budding yeast (Saccharomyces cerevisiae) that confer growth defects.

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Last Updated: Jul 13, 2026

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

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Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System

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Complementation of these yeast mutations by expressing cDNA libraries from heterologous organisms, primarily human.

Deducing gene function based on the successful complementation of specific yeast mutations by human genes.

Main Results:

Successful identification of human genes with conserved functions by complementing yeast mutations.
Demonstrated efficacy of yeast complementation in fields seemingly unrelated to yeast biology, such as apoptosis and steroid receptor signaling.
Established yeast as a versatile platform for functional gene discovery across diverse biological processes.

Conclusions:

Yeast gene complementation is a robust and widely applicable method for discovering gene functions.
This technique facilitates the identification of evolutionarily conserved pathways and molecular mechanisms.
The study underscores the power of model organisms in unraveling complex biological processes in higher eukaryotes.