Related Experiment Video
Updated: Jul 13, 2026

13:23
A Protocol for Collecting and Constructing Soil Core Lysimeters
Published on: June 6, 2016
Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase
K F Knowlton1, M S Taylor, S R Hill
1Virginia Polytechnic Institute and State University, Blacksburg 24061, USA. Knowlton@vt.edu
Journal of Dairy Science
|August 19, 2007
Summary
Supplementing dairy cow diets with an exogenous phytase and cellulase enzyme blend reduced fecal nutrient excretion. This enzyme formulation improved nutrient digestibility and decreased the excretion of dry matter, fiber, nitrogen, and phosphorus in lactating cows.
Area of Science:
- Animal Science
- Nutritional Biochemistry
- Ruminant Nutrition
Background:
- Dietary nutrient management is crucial for dairy cow health and environmental sustainability.
- Exogenous enzymes, such as phytase and cellulase, are explored to enhance nutrient utilization.
- Reducing nutrient excretion minimizes environmental impact from livestock operations.
Purpose of the Study:
- To evaluate the impact of an exogenous phytase and cellulase enzyme formulation on nutrient digestibility.
- To assess the effect of the enzyme blend on nutrient excretion in lactating Holstein cows.
Main Methods:
- Twenty-four Holstein cows were assigned to diets with or without a cellulase-phytase blend for 31 days.
- Diets consisted of corn and alfalfa silage, with specific forage, protein, and fiber content.
- Nutrient digestibility and fecal excretion were measured using acid detergent lignin as an internal marker.
Main Results:
- The enzyme formulation did not affect body weight or milk yield.
- Cows fed the enzyme blend showed reduced fecal excretion of dry matter, neutral detergent fiber, and acid detergent fiber.
- Fecal nitrogen and phosphorus excretion were significantly lower in cows receiving the enzyme formulation.
- Apparent digestibility of dry matter, fiber components, and nitrogen tended to increase with enzyme supplementation.
Conclusions:
- Supplementation with an exogenous phytase and cellulase enzyme formulation effectively reduced fecal nutrient excretion in lactating dairy cows.
- The enzyme blend shows potential for improving nutrient utilization and mitigating environmental pollution from dairy farming.
- Further research could explore optimal inclusion rates and long-term effects of such enzyme formulations.
Related Concept Videos
Amino Acid Catabolism
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
Bioplastics
Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...

