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Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Prokaryotes02:35

Replication in Prokaryotes

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Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
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Related Experiment Video

Updated: Jul 13, 2026

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

Coronavirus replication does not require the autophagy gene ATG5.

Zijiang Zhao1, Larissa B Thackray, Brian C Miller

  • 1Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.

Autophagy
|August 19, 2007
PubMed
Summary

Autophagy, a cellular process involving ATG5, can be antiviral or support viral replication. This study found ATG5 and autophagy are not required for murine hepatitis virus (MHV) replication or release.

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Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Related Experiment Videos

Last Updated: Jul 13, 2026

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

Study of Protein-protein Interactions in Autophagy Research
14:08

Study of Protein-protein Interactions in Autophagy Research

Published on: September 9, 2017

Area of Science:

  • Cell Biology
  • Virology
  • Immunology

Background:

  • Macroautophagy (autophagy) is a cellular degradation process requiring ATG5, delivering cytoplasmic components to lysosomes.
  • Autophagy's role in viral infections is debated, with evidence suggesting both antiviral and pro-viral functions.
  • RNA virus replication often involves cytoplasmic membrane rearrangements, making autophagy a potential factor.

Purpose of the Study:

  • To investigate the role of ATG5 and the autophagic pathway in murine hepatitis virus (MHV) replication.
  • To determine if ATG5 is essential for MHV replication or release in relevant cell types.

Main Methods:

  • Utilized bone marrow-derived macrophages (BMMphi) lacking ATG5 via Cre-recombinase mediated gene deletion.
  • Employed primary low-passage murine ATG5(-/-) embryonic fibroblasts (pMEFs).
  • Assessed MHV replication and release in ATG5-deficient cells compared to controls.

Main Results:

  • Murine hepatitis virus (MHV) replication occurred efficiently in BMMphi lacking ATG5.
  • MHV replication and release were not impaired in primary murine ATG5(-/-) embryonic fibroblasts.
  • These findings indicate ATG5 is dispensable for MHV propagation.

Conclusions:

  • ATG5 is not required for murine hepatitis virus (MHV) replication or release.
  • An intact autophagic pathway is not essential for MHV replication or release.
  • The study challenges the hypothesis that autophagy is necessary for RNA virus replication involving membrane rearrangements.