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Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Stem Cell Therapy for Tissue Regeneration

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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iPS Cell Differentiation

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Jul 11, 2026

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Published on: January 4, 2015

Gene therapy approaches for stem cell protection.

J S Greenberger¹

¹Department of Radiation Oncology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213-2532, USA. greenbergerjs@upmc.edu

|August 19, 2007

Summary

Antioxidant gene therapy using manganese superoxide dismutase (SOD) can protect organs from radiation damage by reducing persistent oxidative stress. This approach shows promise for preventing acute and late toxicities in stem cell populations.

Area of Science:

Radiation biology
Stem cell biology
Gene therapy

Background:

Cytotoxic exposure, including ionizing radiation, damages stem cell populations and their supportive microenvironments.
Oxidative stress, mediated by radical oxygen species, persists long after irradiation in various organs.
The microenvironment plays a crucial role in protecting stem cells from radiation-induced damage.

Purpose of the Study:

To review the role of antioxidant gene therapy in protecting stem cells and their microenvironments from radiation damage.
To explore the mechanisms by which manganese superoxide dismutase (SOD) confers radiation protection.
To discuss novel strategies for stem cell protection based on current evidence.

Main Methods:

Review of existing literature on radiation-induced oxidative stress and antioxidant gene therapy.

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Analysis of studies using manganese superoxide dismutase plasmid liposomes for organ-specific radiation protection.

Examination of evidence regarding the contribution of microenvironmental transgene expression to protection.

Main Results:

Antioxidant gene therapy with manganese superoxide dismutase (SOD) plasmid liposomes demonstrates organ-specific radiation protection.
This therapy delays or prevents both acute and late radiation toxicities.
Transgene expression of SOD within the organ microenvironment is a significant factor in the protective mechanism.

Conclusions:

Manganese superoxide dismutase (SOD) gene therapy offers a promising approach for mitigating radiation damage to stem cell populations.
Understanding the role of the microenvironment in radiation protection can guide the development of enhanced therapeutic strategies.
Further research into novel approaches for stem cell protection is warranted.