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Voltammetry: Overview

Voltammetry: Overview

Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...

Voltammetric Techniques: Pulse Voltammetry

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...

Voltammetry: Factors Affecting Measurements

Voltammetry: Factors Affecting Measurements

A current produced due to the redox reactions of the analyte at the working and auxiliary electrodes is called a faradaic current. The reaction can be divided into two types. The current generated due to the reduction of the analyte is called cathodic current, and it carries a positive charge. In contrast, the current produced by analyte oxidation is known as an anodic current, and it has a negative charge. The applied potential at the working electrode determines the faradaic current flow, and...

Voltammetric Techniques: Linear-Scan (E vs Time)

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

Voltammetry: Stripping Methods

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...

Voltammetric Techniques: Cyclic Voltammetry

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...

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Related Experiment Video

Updated: Jul 13, 2026

Application of Voltage in Dynamic Light Scattering Particle Size Analysis

Application of Voltage in Dynamic Light Scattering Particle Size Analysis

Published on: January 24, 2020

Soft landed protein voltammetry.

Federico Pepi¹, Andreina Ricci, Alessandra Tata

¹Università degli studi di Roma La Sapienza, Dipartimento di Studi di Chimica e Tecnologia delle Sostanze Biologicamente Attive, Piazzale Aldo Moro 5, 00185 Roma, Italy. Federico.Pepi@uniroma1.it

Chemical Communications (Cambridge, England)

|August 19, 2007

Summary

A novel soft landed protein voltammetry (SLPV) method enables electrode functionalization with active molecules. This technique merges ion soft landing and voltammetry for applications in molecular electronics and protein chips.

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Last Updated: Jul 13, 2026

Application of Voltage in Dynamic Light Scattering Particle Size Analysis

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Area of Science:

Electrochemistry
Surface Science
Biotechnology

Background:

Electrode functionalization is crucial for biosensor development.
Existing methods for immobilizing biomolecules can be complex or inefficient.
There is a need for precise and gentle methods to attach proteins to surfaces.

Purpose of the Study:

To introduce a new experimental technique called soft landed protein voltammetry (SLPV).
To demonstrate the coupling of ion soft landing with voltammetry for protein immobilization.
To explore the potential applications of this method in advanced fields.

Main Methods:

Developed a novel method combining ion soft landing and voltammetry.
Utilized soft landing to deposit proteins onto an electrode surface.
Employed voltammetric measurements to confirm protein activity and surface functionalization.

Main Results:

Successfully functionalized electrode surfaces with biologically active proteins using SLPV.
Demonstrated the capability of the technique to preserve protein function.
Showcased the versatility of SLPV for creating functionalized surfaces.

Conclusions:

Soft landed protein voltammetry (SLPV) offers a precise and effective approach for electrode modification.
This method facilitates the development of novel biosensors and protein-based devices.
SLPV opens new avenues in molecular electronics and the creation of protein chips.