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Identifying Protein-protein Interaction Sites Using Peptide Arrays
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Published on: November 18, 2014

CD147 and its interacting proteins in cellular functions.

Jian-Li Jiang1, Juan Tang

  • 1Cell Engineering Research Centre and Department of Cell Biology, State Key Laboratory of Cancer Biology, the Fourth Military Medical University, Xi'an 710032, China. jiangjl@fmmu.edu.cn

Sheng Li Xue Bao : [Acta Physiologica Sinica]
|August 19, 2007
PubMed
Summary
This summary is machine-generated.

CD147, a transmembrane glycoprotein, is highly expressed on epithelial cancer cells. Its structure suggests involvement in protein-protein interactions, mediating diverse cellular functions through interactions with various molecules.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • CD147 (basigin, EMMPRIN) is a transmembrane glycoprotein expressed on various epithelial cells.
  • Expression is significantly enriched on epithelial-derived cancer cells, including lung, breast, and hepatoma.
  • CD147 possesses a structure with two IgSF domains, a transmembrane sequence, and a cytoplasmic tail, suggesting roles in protein interactions.

Purpose of the Study:

  • To investigate the structural features of CD147 and its potential roles in cellular functions.
  • To identify interacting molecules (ligands or receptors) of CD147.
  • To understand the implications of CD147's interactions in cancer biology.

Main Methods:

  • Analysis of CD147's structural domains (IgSF, transmembrane, cytoplasmic).
  • Adhesion assays to study cell-surface interactions.
  • Coimmunoprecipitation studies to identify interacting proteins.
  • Literature review of existing studies on CD147 function.

Main Results:

  • CD147's structure, including its charged transmembrane residue and cytoplasmic domain, points to its involvement in protein-protein interactions.
  • While the 3D structure is unavailable, studies suggest interactions with integrins, cyclophilins, and MCTs.
  • These interactions are proposed to mediate a broad spectrum of cellular functions.

Conclusions:

  • CD147 is a key mediator of cellular functions through protein-protein interactions.
  • Further research into CD147's interacting partners and 3D structure is warranted.
  • CD147 represents a potential therapeutic target in epithelial cancers due to its enrichment and functional roles.