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Updated: Jul 13, 2026

Lethality Bioassay Using Artemia salina L.
Published on: October 11, 2022
Acute and sub-lethal toxicity of three POEA surfactant formulations to Daphnia magna
John M Brausch1, Blake Beall, Philip N Smith
1The Institute of Environmental and Human Health, Department of Environmental Toxicology, Texas Tech University, Lubbock, TX 79409, USA. john.brausch@ttu.edu
Abstract:
Polyethoxylated tallowamine (POEA) is a non-ionic surfactant used in many herbicide formulations to increase the ability of active ingredients to penetrate leaf cuticles. However, it has also been shown to disrupt respiratory membranes in aquatic organisms. In this study, Daphnia magna was used to examine the lethal and sub-lethal toxicity of three POEA formulations consisting of 5:1, 10:1, and 15:1 average oxide:tallowamine. The formulation consisting of 10:1 was the most acutely toxic with a 48-h LC50 value of 97.0 microg/L and 15:1 was least toxic at 849.4 microg/L. All formulations inhibited growth at concentrations between 100 and 500 microg/L.
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