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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jul 13, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multicolor super-resolution imaging with photo-switchable fluorescent probes.

Mark Bates¹, Bo Huang, Graham T Dempsey

¹School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)

|August 19, 2007

Summary

Researchers developed new photo-switchable fluorescent probes for multicolor super-resolution microscopy. This breakthrough enables high-resolution imaging of biological samples, visualizing molecular interactions with unprecedented detail.

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Last Updated: Jul 13, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

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Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

Optics and Photonics
Biotechnology
Molecular Biology

Background:

Far-field optical nanoscopy achieves 20-50 nm resolution.
Multicolor super-resolution imaging presents significant challenges.

Purpose of the Study:

Introduce novel photo-switchable fluorescent probes.
Demonstrate multicolor stochastic optical reconstruction microscopy (STORM).

Main Methods:

Developed photo-switchable probes with reporter and activator components.
Utilized combinatorial pairing for diverse probe colors.
Employed iterative, color-specific activation for localization.

Main Results:

Achieved multicolor STORM imaging with 20-30 nm resolution.
Successfully imaged DNA models and mammalian cells.
Demonstrated nanometer-scale visualization of molecular interactions.

Conclusions:

This technique overcomes multicolor super-resolution imaging challenges.
Enables direct visualization of molecular interactions at the nanometer scale.
Advances capabilities in nanoscopy and molecular biology research.