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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays

Mayka Sanchez1, Bruno Galy, Matthias W Hentze

  • 1Molecular Medicine Partnership Unit (MMPU), Im Neuenheimer Feld 153, 69120 Heidelberg, Germany.

Nature Protocols
|August 19, 2007
PubMed

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...