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Updated: Jul 13, 2026

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
Published on: September 26, 2016
Determining if a system is heterogeneous: the analysis of single molecule rotational correlation functions and their
Chia-Yin Joyce Wei1, Chun-Yaung Lu, Yeon Ho Kim
1Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station, Austin, TX 78712, USA.
Abstract:
Single molecule spectroscopy can be utilized to measure distributions of individual molecular properties that may be averaged out in the ensemble measurement. For example, complex dynamics in disordered systems can be investigated by observing single molecule rotations via fluorescence spectroscopy. The rotational time of a single transient can be calculated from the correlation function of the reduced linear dichroism signal which fluctuates over time as the molecule reorients in its surroundings. Distributions of rotational time constants can be used to characterize the heterogeneity of molecular environments in the material. This paper reviews some theoretical studies on (1) the high numerical aperture effects on the final correlation function, and how it can be related to optical anisotropy decays in a bulk measurement; (2) the statistical errors resulting from the finite observation length that will propagate into distributions of rotational times. These lead to the discussions on how to interpret correctly the distribution of properties measured from a set of single molecule data, and to determine if in fact the system is heterogeneous.
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