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Plasmid-encoded determinants for bacteriocin production and immunity in a Lactococcus lactis strain and purification
A Dufour1, D Thuault, A Boulliou
1URA 256, Laboratoire de Génétique Moléculaire, Université de Rennes, France.
Journal of General Microbiology
|October 1, 1991
Abstract:
Lactococcin, a bacteriocin produced by Lactococcus lactis subsp. lactis ADRIA 85LO30, was purified as a 2.3-2.4 kDa peptide. Six non-bacteriocin-producing (Bac-) and non-immune (Imm-) strains were isolated after curing experiments. These strains had in common the loss or modification of two plasmids: pOS4 (32 kb) and pOS5 (70 kb). By comparing pOS5 and several modified plasmids, a DNA region from pOS5 of about 10 kb, which was necessary for wild-type bacteriocin production and immunity, was identified.