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Updated: Jul 13, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23
Adriano Augusto Melo de Mendonça1, Pedro Paulo Chaves Souza, Josimeri Hebling
1Graduate Student, Department of Restorative Dentistry, School of Dentistry, University of São Paulo State, Araraquara, São Paulo, Brazil.
Objective:
The present study evaluated the cytotoxic effects of hard setting applied on the odontoblastlike cells MDPC-23.
Study Design:
Eighty round-shaped samples were prepared with the following experimental materials: calcium hydroxide, Vitrebond, RelyX Luting, and RelyX Unicem. The samples were placed in serum-free culture medium and incubated for 24 hours or 7 days at 37 degrees C with 5% CO2 and 95% air. The odontoblast cells were plated in the wells and incubated for 72 hours. After this period, the complete culture medium was replaced by the extracts obtained from every sample, and the methyltetrazolium assay was carried out to evaluate the cell metabolism.
Results:
For the 24-hour period, the experimental materials calcium hydroxide, Vitrebond, RelyX Luting, and RelyX Unicem decreased the cell metabolic activity by 91.52%, 81.14%, 78.17%, and 2.64%, respectively. For the 7-day period, calcium hydroxide, Vitrebond, RelyX Luting, and RelyX Unicem decreased the metabolic activity of the MDPC-23 cells by 91.13%, 87.27%, 79.04%, and 10.51%, respectively.
Conclusion:
RelyX Unicem presented the lowest cytopathic effects to the cultured odontoblast cell line.
