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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

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Related Experiment Video

Updated: Jul 13, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Does distance matter? Variations in alternative 3' splicing regulation.

Martin Akerman1, Yael Mandel-Gutfreund

  • 1The Faculty of Biology, Technion-Israel Institute of Technology, Haifa Israel 32000.

Nucleic Acids Research
|August 21, 2007
PubMed
Summary

Alternative splicing creates protein diversity. Researchers identified unique features of alternative 3' splice sites conserved in humans and mice, enabling discrimination from constitutive sites.

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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Related Experiment Videos

Last Updated: Jul 13, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing is a key mechanism for generating protein diversity in humans.
  • This process involves alternative exon skipping or selection of splice sites (5' or 3').

Purpose of the Study:

  • To analyze sequence and evolutionary features of conserved alternative 3' splice sites between human and mouse genomes.
  • To distinguish alternative splicing events from constitutive splicing based on splice site properties and distances.
  • To investigate the regulatory mechanisms influencing alternative splice site selection.

Main Methods:

  • Comparative analysis of conserved alternative 3' splice sites in human and mouse genomes (3-100 nucleotides).
  • Identification of unique sequence features, including overlapping polypyrimidine tracts.
  • Application of machine-learning algorithms to discriminate alternative from constitutive 3' splice sites.

Main Results:

  • Alternative and constitutive splicing events exhibit distinct properties influenced by splice site distance.
  • A high occurrence of overlapping polypyrimidine tracts was observed at alternative 3' splice sites.
  • Machine learning successfully differentiated true alternative 3' splice sites from constitutive ones.

Conclusions:

  • Unique features of introns flanking alternative splice sites suggest a regulatory role in splice site selection.
  • The distance between competing splice sites appears to influence the splice site selection process.
  • Conserved sequence and evolutionary characteristics provide insights into the regulation of alternative splicing.